LOCUS pDONR™/Zeo DISCLAIMER Certain terms are trademarks or registered trademarks of Invitrogen Corporation. See "Intellectual Property" in the Help file for more information. FEATURES Location/Qualifiers misc_feature complement(268..295) /note="rrnB T2 transcription termination sequence (c)" misc_feature complement(427..470) /note="rrnB T1 transcription termination sequence (c)" primer_bind 537..552 /note="M13 Forward (-20) priming site" misc_recomb 570..668 /label=attL1 /note="attL1" misc_recomb complement(3761..3857) /label=attL2 /note="attL2 (c)" misc_signal complement(3872..3891) /note="T7 Promoter/priming site (c)" primer_bind 3899..3915 /note="M13 Reverse priming site" gene 4028..4837 /note="Kanamycin resistance gene" rep_origin 4958..5631 /note="pUC origin" vector join(3769..5634,1..651) /source="pDONR%99221" /type="Donor Vector" misc_feature 669..691 /note="TEV site" source 692..3754 /organism="Homo sapiens" /mol_type="mRNA" /db_xref="taxon:9606" /clone="MGC:22921 IMAGE:4865031" /tissue_type="Colon, adenocarcinoma" /clone_lib="NIH_MGC_15" /lab_host="DH10B-R" /note="Vector: pOTB7" gene 692..3754 /gene="OGT" /gene_synonym=FLJ23071 /gene_synonym=HRNT1 /gene_synonym=MGC22921 /gene_synonym=O-GLCNAC /db_xref="GeneID:8473" /db_xref="HGNC:8127" /db_xref="MIM:300255" CDS 692..3754 /dnas_title="O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)" /gene="OGT" /gene_synonym=FLJ23071 /gene_synonym=HRNT1 /gene_synonym=MGC22921 /gene_synonym=O-GLCNAC /codon_start=1 /product="O-linked N-acetylglucosamine (GlcNAc) transferase (UDP-N-acetylglucosamine:polypeptide-N-acetylglucosaminyl transferase)" /protein_id="AAH14434.1" /db_xref="GI:15680175" /db_xref="GeneID:8473" /db_xref="HGNC:8127" /db_xref="MIM:300255" /translation="MASSVGNVADSTGLAELAHREYQAGDFEAAERHCMQLWRQEPDN TGVLLLLSSIHFQCRRLDRSAHFSTLAIKQNPLLAEAYSNLGNVYKERGQLQEAIEHY RHALRLKPDFIDGYINLAAALVAAGDMEGAVQAYVSALQYNPDLYCVRSDLGNLLKAL GRLEEAKACYLKAIETQPNFAVAWSNLGCVFNAQGEIWLAIHHFEKAVTLDPNFLDAY INLGNVLKEARIFDRAVAAYLRALSLSPNHAVVHGNLACVYYEQGLIDLAIDTYRRAI ELQPHFPDAYCNLANALKEKGSVAEAEDCYNTALRLCPTHADSLNNLANIKREQGNIE EAVRLYRKALEVFPEFAAAHSNLASVLQQQGKLQEALMHYKEAIRISPTFADAYSNMG NTLKEMQDVQGALQCYTRAIQINPAFADAHSNLASIHKDSGNIPEAIASYRTALKLKP DFPDAYCNLAHCLQIVCDWTDYDERMKKLVSIVADQLEKNRLPSVHPHHSMLYPLSHG FRKAIAERHGNLCLDKINVLHKPPYEHPKDLKLSDGRLRVGYVSSDFGNHPTSHLMQS IPGMHNPDKFEVFCYALSPDDGTNFRVKVMAEANHFIDLSQIPCNGKAADRIHQDGIH ILVNMNGYTKGARNELFALRPAPIQAMWLGYPGTSGALFMDYIITDQETSPAEVAEQY SEKLAYMPHTFFIGDHANMFPHLKKKAVIDFKSNGHIYDNRIVLNGIDLKAFLDSLPD VKIVKMKCPDGGDNADSSNTALNMPVIPMNTIAEAVIEMINRGQIQITINGFSISNGL ATTQINNKAATGEEVPRTIIVTTRSQYGLPEDAIVYCNFNQLYKIDPSTLQMWANILK RVPNSVLWLLRFPAVGEPNIQQYAQNMGLPQNRIIFSPVAPKEEHVRRGQLADVCLDT PLCNGHTTGMDVLWAGTPMVTMPGETLASRVAASQLTCLGCLELIAKNRQEYEDIAVK LGTDLEYLKKVRGKVWKQRISSPLFNTKQYTMELERLYLQMWEHYAAGNKPDHMIKPV EVTESA" misc_feature 692..3754 /note="OGT coding region" ORIGIN 1 CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA 61 TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA 121 GCGCCCAATA CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA 181 CGACAGGTTT CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATA CGCGTACCGC 241 TAGCCAGGAA GAGTTTGTAG AAACGCAAAA AGGCCATCCG TCAGGATGGC CTTCTGCTTA 301 GTTTGATGCC TGGCAGTTTA TGGCGGGCGT CCTGCCCGCC ACCCTCCGGG CCGTTGCTTC 361 ACAACGTTCA AATCCGCTCC CGGCGGATTT GTCCTACTCA GGAGAGCGTT CACCGACAAA 421 CAACAGATAA AACGAAAGGC CCAGTCTTCC GACTGAGCCT TTCGTTTTAT TTGATGCCTG 481 GCAGTTCCCT ACTCTCGCGT TAACGCTAGC ATGGATGTTT TCCCAGTCAC GACGTTGTAA 541 AACGACGGCC AGTCTTAAGC TCGGGCCCCA AATAATGATT TTATTTTGAC TGATAGTGAC 601 CTGTTCGTTG CAACACATTG ATGAGCAATG CTTTTTTATA ATGCCAACTT TGTACAAAAA 661 AGCAGGCTct gaaaacttgt actttcaagg cgagttggca catcgagaat atcaggcagg 721 agattttgag gcagctgaga gacactgcat gcagctctgg agacaagagc cagacaatac 781 tggtgtgctt ttattacttt catctataca cttccagtgt cgaaggctgg acagatctgc 841 tcactttagc actctggcaa ttaaacagaa cccccttctg gcagaagctt attcgaattt 901 ggggaatgtg tacaaggaaa gagggcagtt gcaggaggca attgagcatt atcgacatgc 961 attgcgtctc aaacctgatt tcatcgatgg ttatattaac ctggcagccg ccttggtagc 1021 agcgggtgac atggaagggg cagtacaagc ttacgtctct gctcttcagt acaatcctga 1081 tttgtactgt gttcgcagtg acctggggaa cctgctcaaa gccctgggtc gcttggaaga 1141 agccaaggca tgttatttga aagcaattga gacgcaaccg aactttgcag tagcttggag 1201 taatcttggc tgtgttttca atgcacaagg ggaaatttgg cttgcaattc atcactttga 1261 aaaggctgtc acccttgacc caaactttct ggatgcttat atcaatttag gaaatgtctt 1321 gaaagaggca cgcatttttg acagagctgt ggcagcttat cttcgtgccc taagtttgag 1381 tccaaatcac gcagtggtgc acggcaacct ggcttgtgta tactatgagc aaggcctgat 1441 agatctggca atagacacct acaggcgggc tatcgaacta caaccacatt tccctgatgc 1501 ttactgcaac ctagccaatg ctctcaaaga gaagggcagt gttgctgaag cagaagattg 1561 ttataataca gctctccgtc tgtgtcccac ccatgcagac tctctgaata acctagccaa 1621 tatcaaacga gaacagggaa acattgaaga ggcagttcgc ttgtatcgta aagcattaga 1681 agtcttccca gagtttgctg ctgcccattc aaatttagca agtgtactgc agcagcaggg 1741 aaaactgcag gaagctctga tgcattataa ggaggctatt cgaatcagtc ctacctttgc 1801 tgatgcctac tctaatatgg gaaacactct aaaggagatg caggatgttc agggagcctt 1861 gcagtgttat acgcgtgcca tccaaattaa tcctgcattt gcagatgcac atagcaatct 1921 ggcttccatt cataaggatt cagggaatat tccagaagcc atagcttctt accgcacggc 1981 tctgaaactt aagcctgatt ttcctgatgc ttattgtaac ttggctcatt gcctgcagat 2041 tgtctgtgat tggacagact atgatgagcg aatgaagaag ttggtcagta ttgtggctga 2101 ccagttagag aagaataggt tgccttctgt gcatcctcat catagtatgc tatatcctct 2161 ttctcatggc ttcaggaagg ctattgctga gaggcacggc aacctgtgct tagataagat 2221 taatgttctt cataaaccac catatgaaca tccaaaagac ttgaagctca gtgatggtcg 2281 gctgcgtgta ggatatgtga gttccgactt tgggaatcat cctacttctc accttatgca 2341 gtctattcca ggcatgcaca atcctgataa atttgaggtg ttctgttatg ccctgagccc 2401 agacgatggc acaaacttcc gagtgaaggt gatggcagaa gccaatcatt tcattgatct 2461 ttctcagatt ccatgcaatg gaaaagcagc tgatcgcatc catcaggatg gaattcatat 2521 ccttgtaaat atgaatggct atactaaggg cgctcgaaat gagctttttg ctctcaggcc 2581 agctcctatt caggcaatgt ggctgggata ccctgggacg agtggtgcgc ttttcatgga 2641 ttatattatc actgatcagg aaacttcgcc agctgaagtt gctgagcagt attccgagaa 2701 attggcttat atgccccaca ctttttttat tggtgatcat gctaatatgt tccctcacct 2761 gaagaaaaaa gcagtcatcg attttaagtc caatgggcac atttatgaca atcggatagt 2821 tctgaatggc atcgacctca aagcatttct tgatagtcta ccagatgtga aaattgtcaa 2881 gatgaagtgt cctgatggag gagacaatgc agatagcagt aacacagctc ttaatatgcc 2941 tgttattcct atgaatacta ttgcagaagc agttattgaa atgattaacc gaggacagat 3001 tcaaataaca attaatggat tcagtattag caatggactg gcaactactc agatcaacaa 3061 taaggctgca actggagagg aggttccccg taccattatt gtaaccaccc gttctcagta 3121 cgggttacca gaagatgcca tcgtatactg taactttaat cagttgtata aaattgaccc 3181 ttctactttg cagatgtggg caaacattct gaagcgtgtt cccaatagtg tactctggct 3241 gttgcgtttt ccagcagtag gagaacctaa tattcaacag tatgcacaaa acatgggcct 3301 gccccagaac cgtatcattt tttcacctgt tgctcctaaa gaggaacacg tcaggagagg 3361 ccagctggct gatgtctgct tggacactcc actctgtaat gggcacacca cagggatgga 3421 tgtcctctgg gcagggaccc ccatggtgac tatgccagga gagactcttg cttctcgagt 3481 tgcagcatcc cagctcactt gcttaggttg tcttgagctt attgctaaaa acagacaaga 3541 atatgaagac atagctgtga agctgggaac tgatctagaa tacctgaaga aagttcgtgg 3601 caaagtctgg aagcaaagaa tatctagccc tctgttcaac accaaacaat acacaatgga 3661 actagagcgg ctctatctac agatgtggga gcattatgca gctggcaaca aacctgacca 3721 catgattaag cctgttgaag tcactgagtc agcaTAGGAC CCAGCTTTCT TGTACAAAGT 3781 TGGCATTATA AGAAAGCATT GCTTATCAAT TTGTTGCAAC GAACAGGTCA CTATCAGTCA 3841 AAATAAAATC ATTATTTGCC ATCCAGCTGA TATCCCCTAT AGTGAGTCGT ATTACATGGT 3901 CATAGCTGTT TCCTGGCAGC TCTGGCCCGT GTCTCAAAAT CTCTGATGTT ACATTGCACA 3961 AGATAAAATA ATATCATCAT GAACAATAAA ACTGTCTGCT TACATAAACA GTAATACAAG 4021 GGGTGTTATG AGCCATATTC AACGGGAAAC GTCGAGGCCG CGATTAAATT CCAACATGGA 4081 TGCTGATTTA TATGGGTATA AATGGGCTCG CGATAATGTC GGGCAATCAG GTGCGACAAT 4141 CTATCGCTTG TATGGGAAGC CCGATGCGCC AGAGTTGTTT CTGAAACATG GCAAAGGTAG 4201 CGTTGCCAAT GATGTTACAG ATGAGATGGT CAGACTAAAC TGGCTGACGG AATTTATGCC 4261 TCTTCCGACC ATCAAGCATT TTATCCGTAC TCCTGATGAT GCATGGTTAC TCACCACTGC 4321 GATCCCCGGA AAAACAGCAT TCCAGGTATT AGAAGAATAT CCTGATTCAG GTGAAAATAT 4381 TGTTGATGCG CTGGCAGTGT TCCTGCGCCG GTTGCATTCG ATTCCTGTTT GTAATTGTCC 4441 TTTTAACAGC GATCGCGTAT TTCGTCTCGC TCAGGCGCAA TCACGAATGA ATAACGGTTT 4501 GGTTGATGCG AGTGATTTTG ATGACGAGCG TAATGGCTGG CCTGTTGAAC AAGTCTGGAA 4561 AGAAATGCAT AAACTTTTGC CATTCTCACC GGATTCAGTC GTCACTCATG GTGATTTCTC 4621 ACTTGATAAC CTTATTTTTG ACGAGGGGAA ATTAATAGGT TGTATTGATG TTGGACGAGT 4681 CGGAATCGCA GACCGATACC AGGATCTTGC CATCCTATGG AACTGCCTCG GTGAGTTTTC 4741 TCCTTCATTA CAGAAACGGC TTTTTCAAAA ATATGGTATT GATAATCCTG ATATGAATAA 4801 ATTGCAGTTT CATTTGATGC TCGATGAGTT TTTCTAATCA GAATTGGTTA ATTGGTTGTA 4861 ACACTGGCAG AGCATTACGC TGACTTGACG GGACGGCGCA AGCTCATGAC CAAAATCCCT 4921 TAACGTGAGT TACGCGTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC 4981 TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT 5041 ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG 5101 CTTCAGCAGA GCGCAGATAC CAAATACTGT TCTTCTAGTG TAGCCGTAGT TAGGCCACCA 5161 CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC 5221 TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA 5281 TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC 5341 GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA 5401 AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG 5461 GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG 5521 ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG 5581 CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTT //